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Literature summary for 1.14.99.67 extracted from

  • Lu, H.; Chanco, E.; Zhao, H.
    CmlI is an N-oxygenase in the biosynthesis of chloramphenicol (2012), Tetrahedron, 68, 7651-7654 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.67

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces venezuelae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme di-iron enzyme, iron content is 1.2-1.4 mol per mol of enzyme Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2 Streptomyces venezuelae
-
 ?
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2 Streptomyces venezuelae DSM 40230
-
 ?
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2 Streptomyces venezuelae
-
 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2 Streptomyces venezuelae DSM 40230
-
 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae F2RB83
-
-
Streptomyces venezuelae DSM 40230 F2RB83
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae ?
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 ?
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae chloramphenicol + phenazine ethosulfate + 2 H2O
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine ethosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 chloramphenicol + phenazine ethosulfate + 2 H2O
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
-
 Streptomyces venezuelae chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
(1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
-
 Streptomyces venezuelae DSM 40230 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
additional information no acceptor: flavin mononucleotide. The reaction shows a biphasic behavior, with a fast step consuming significant amounts of substrate within 15 s, and a slower step afterward Streptomyces venezuelae ?
-
-
additional information no acceptor: flavin mononucleotide. The reaction shows a biphasic behavior, with a fast step consuming significant amounts of substrate within 15 s, and a slower step afterward Streptomyces venezuelae DSM 40230 ?
-
-

Synonyms

Synonyms Comment Organism
cmlI
-
 Streptomyces venezuelae

Cofactor

Cofactor Comment Organism Structure
additional information no acceptor: flavin mononucleotide Streptomyces venezuelae

General Information

General Information Comment Organism
physiological function CmlI is a non-heme di-iron enzyme catalyzing N-oxygenation of an amine group, one of the steps in the biosynthesis of the antibiotic chloramphenicol Streptomyces venezuelae